Physical exercise induces biochemical changes in human body that modify metabolites in blood, saliva and other body fluids. Saliva biochemical analysis is rapidly developing as a tool for the assessment of physiological biomarkers of sports training. The aim of this study was to investigate changes in antioxidant status after performing two loading patterns, i.e. pyramid (PLP) and inverse pyramid (RPLP). In practice, 36 young women athletes (height 162.7 ± 9.5 cm, weight 55.8 ±5 kg, fat 21.8 ± 5.5, BMI 21.1 ± 2.2) voluntarily participated in the study. On two consecutive days (one week apart) they performed 6 different movements in resistance exercise protocols with two loading patterns, i.e. pyramid (PLP) and inverse pyramid (RPLP). Their un-stimulated saliva was then donated before and 5 minutes after exercise. Alteration in activity of superoxide dismutase (SOD), radical scavenging activity on 2, 2-diphenyl-2-picrylhydrazyl hydrate (DPPH) and concentration of uric acid (UA) was then investigated.
The results showed that after exercise, SOD activity was significantly higher in both PLP and RPLP when compared to their values at rest (p<0.05). Uric acid values increased significantly in both resistance exercise systems (p<0.05). In the case of radical scavenging activity (%DPPH), both PLP and RPLP showed significant increases 5 minutes after exercise sessions compared to pre-exercise (p<0.05).
resistance exercise systems, salivary uric acid, superoxide dismutase
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